Methods In Plant Biotechnology

  • Title : Methods In Plant Biotechnology
  • Author : K R Koundal
  • ISBN 13 : 9789390591725
  • Year : 2022
  • Language : English
  • Binding : Hardbound
  • MRP : Rs 2995
  • Selling Price : Rs 2396
  • Discount : 20%
Add to Cart

Description

The book deals with some of the important techniques of recombinant DNA technology including ; isolation of plasmids and transformation of bacteria, isolation and characterization of nucleic acids, construction and screening of gene libraries, DNA sequencing, RFLP and RAPD gene mapping, genetic transformation and molecular analysis of the transgenic plants. The book includes protocols of some the important techniques currently in use in the field of plant biotechnology. The book also includes methods used in RFPL and RAPD analysis and a practical example of actual research problem in the field of plant genetic engineering. A glossary of important terms is also included which will be helpful to understand the subject matter. The preparation of common buffers and reagents used in the different protocols is included in the appendix. Contents: 1. Background - Plant Biotechnology 2. Isolation, Restriction and Agarose Gel Electrophoresis of E.coli Plasmid DNA 3. Transformation of Bacterial Cells 4. Preparation of Bacterophage? DNA 5. Isolation and Purification of Genomic DNA from Plants 6. Isolation of Total RNA and in vitro Translation of Poly (A)+RNA 7. Isolation of and Analysis of Polyribosomes and Ribosomal Proteins 8. Preparation of a Probe: Radioactive Labeling of Nucleic Acids 9. Southern and Northern Blotting and Hybridization 10. Construction of Genomic Library 11. Screening of Genomic Library 12. Construction and Screening of cDNA Library 13. PCR and RT-PCR Methods 14. M13 Cloning for Di-deoxy DNA Squencing 15. DNA Sequencing Methods 16. Isolation and in vitro Culture of Protoplasts 17. Methods of Genetic Transformation of Plants 18. Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD) Analysis 19. Development and Characterization of Transgenic Crop Plants