A Handbook of Basics on Clinical and Applied Ocular Microbiology Optometrists are healthcare professionals who provide primary vision care ranging from sight testing and correction to the diagnosis, treatment, and management of vision changes, which involves eye exams and vision tests, detecting certain eye abnormalities and prescribing medications for certain eye diseases and infections. The course on ocular microbiology is prescribed to understand the basic and applied aspects of microbiology so that can characterize the bacteria, fungi, viruses, protozoa, and parasites which can infect the lids, conjunctiva, and cornea and how they may be influenced by the wear of contact lenses. The objective of the book is to prepare the students to gain essential knowledge about basic microbiology like the classification of microbes and their characteristics. It focuses to cover the principles of sterilization and disinfection in hospital and ophthalmic practice. It explains the pathogenesis of the diseases caused by microorganisms in the human body with reference to eye infections and disease. The subject matter has been planned in a systematic manner for understanding the basic principles of diagnostic ocular microbiology, inflammation, and effective treatment using simple language, clear illustrations and unto date information. The textbook covering the entire width of the prescribed syllabus into seven sections and twenty chapters: The sections focus on Microbiology – Introduction, classification and physiology and control of growth; Bacteriology; Mycology; Virology; Parasitology; Diagnosis of ocular infections; Diagnostic tools, and techniques; Ocular Disease and its manifestations and a dedicated chapter on the WHO Priority Pathogens List for R&D updates. Contents.. Section 1: Introduction to Microbiology Introduction to Microbiology Morphology and Physiology of Microorganisms Control of Microbial Growth (Sterilization and Disinfection) Microbes Versus Human Interaction Section 2: Ocular Bacteriology Ocular Bacteriology Cocci of Ocular Interest Bacilli of Ocular Interest Section 3: Ocular Mycology Ocular Mycology Fungi of Ocular Interest Section 4: Ocular virology Ocular Virology Viruses of Ocular Interest Virus Associated with Eye Complications Section 5: Ocular Parasitology Parasitology Ocular Parasites Section 6: Diagnosis for Ocular Infections Diagnosis for Ocular Infections Diagnostics Tools and Technique for Ocular Infections Section 7: Ocular Disease and Manifestation Ocular Disease Ocular Manifestations Ocular Defense and Mechanisms of Various Antibiotics WHO Priority Pathogens List For R&D Bibliography About the Author: Machiavelli Singh was born in Barnala, Punjab. He obtained his M.Sc. (2001) and Ph.D. (2010) in Biotechnology from Thapar Institute of Engineering & Technology, Patiala. He started his research career as a UNDP fellow under the ITSAP project. Machiavelli joined academics in 2004 as Lecturer and has contributed extensively to the methodologies of biotechnological teaching for last 18 years mentoring UG and PG students in reputed institutions of Kurukshetra University and with the Faculty of Technology & Sciences at LPU. In 2012 he joined Amity University Haryana with many active roles as Research Coordinator; Adjunct Faculty, Amity Institute of Integrative Sciences & Health (AIISH), Visiting Faculty, and Amity Medical School. He is a qualified WIPO alumnus of the IPR and EyeTeach program (BHVI – Australia) and WHO Competency-Based Learning program 2020. His passion for research in Microbial Biotechnology developed special interests in Molecular Markers & Diagnostics, Bioprocessing, AgroBio & IPR. He is mentoring research scholars and managing running grants of Rs 28.45 million as Co-PI in the DBT BUILDER program (2022) and Coordinator, DST – State Skill Vigyan Program (2022). He has 40+ publications (20+ peer-reviewed papers and 20+ book chapters/articles) to his credit along with five authored/edited books. He is a reviewer for many Agri and Bio-Research Journals. He is a board member of AASF-IFSDAA in Germany and a dynamic observer of its international operations. He coordinates MGIRI, MSME and LSSSDC activities under MOUs toward youth skilling on startups/industrialization. He is an active member of SSARM and SBCI. Being organizing secretary, he hosted the 90th SBCI-2021 meeting. Dr Singh is a well-recognised collaborator on an international science network in the EU, US and Canada. He is an onboard advisor and consultant to NVK Diagnostics Research Centre, NextGen Invitro Diagnostics (NGIVD) and Floritech Organo Industries.